




New Sanger Centre Naming Scheme
- Filename
-
sanger_names_new.p4t
- Description
-
This scheme extracts information from sequence names by assuming that they
adhere to the new-style (~1997) Sanger Centre naming scheme. This is explained
clearly on the Sanger Centre's web pages at
http://www.sanger.ac.uk/Software/sequencing/
The information extracted consists of the template name, primer type and chemistry information. The format of a reading name is as follows.
<template_name>
.
<strand><primer><chemistry>The strand, primer and chemistry fields are each one character long and are mandatory. They are defined by the following tables.
- Strand
- Description
p
- Forward, double stranded template.
q
- Reverse, double stranded template.
r
- Reverse, single stranded template.
s
- Forward, single stranded template.
- Primer
- Description
1
- Universal primer (end of insert).
2
- Custom primer.
- Conditions
- Description
t
- standard (ABI) terminator
d
- dRhodamine terminator
p
- standard (ABI) primer
e
- energy transfer primer
b
- big dye primer
c
- big dye terminator
l
- licor
For example "
U16F10.p1t
" is a forward standard (ABI) terminator sequence from the double stranded template namedU16F10
using the universal primer. - Configuration section
-
[naming_scheme]
- Configuration elements
-
PR_com
,TN_com
,CH_com
.





This page is maintained by staden-package. Last generated on 25 April 2003.
URL: http://www.mrc-lmb.cam.ac.uk/pubseq/manual/pregap4_unix_52.html