CSC

Old Sanger Centre Naming Scheme

Filename

sanger_names_old.p4t

Description

This scheme extracts information from sequence names by assuming that they adhere to the old-style Sanger Centre naming scheme. The information extracted consists of the template name, primer type and chemistry information. The format of a reading name is as follows.

<template_name>.<strand><primer><conditions><repetition>

In the above, strand and primer are each one character long and are defined according to the following tables. Conditions can be 0, 1 or 2 characters indicating none, one or two sequencing conditions. Repetitions is optional and is used purely for creating unique names when resequencing a template with the same strand, primer and conditions as a previous sequencing reaction.

Strand

Description

s, f

Forward, single stranded template.

r

Reverse, single stranded template.

p

Forward, double stranded template.

q

Reverse, double stranded template.

Primer

Description

1

Universal primer (end of insert).

2, 3, etc

Custom primer.

Conditions

Description

t

Dye terminator chemistry.

l

Long gel

Repetition

Description

a, b, c, ...

Any letter except l and t

For example "U16F10.p1t" is a forward dye-terminator sequence from the double stranded template named U16F10 using the universal primer.

Configuration section

[naming_scheme]

Configuration elements

PR_com, TN_com, CH_com.